Truman College1, Biology Department, Chicago, IL 60640 Truman Colllege2, Biology Department, Chicago, IL 60640
There is evidence that various nutritional antioxidants are able to protect the body against various diseases such as cancer. However, it is unclear the amount of protection at the cellular level. This experiment will examine the degree of protection provided to cells by specific nutritional antioxidants following hydrogen peroxide. Two experiments, plating efficiency and the growth curve were performed. Cells from the CHO-K1 cell line were used for the experiments. For the plating efficiency experiment cells were observed to have several colonies after eight days. These results led to the conclusion that cells begin to divide on day one and quickly divide after 24 hours. For the growth curve experiment, counting of the mammalian cells occurred every 24 hours for a total of ten days. Cells were observed in the different phases. It was found that the non-treated CHO-K1 cells had reached the lag phase, log phase, stationary, and when they had become over grown and stopped dividing this resulted in the death phase. This experiment is ongoing, currently a continuation of this experiment is underway where the growth curve and plating efficiency protocols will be performed on CHO-K1 cells that have been treated with hydrogen peroxide and several extracts containing antioxidants to monitor their effects on the cells.
[Abstract (DOC)]