Saginaw Valley State University, Biology, University Center, MI 48710
Zebra mussels are an invasive species introduced into the Great Lakes from Europe in 1986. Since then, they have spread aggressively throughout the eastern United States and have had a significant impact on Great Lakes ecology and biodiversity. Samples have been taken from many sites throughout the Great Lakes in order to assess genetic diversity and population substructure in Michigan. Tissue from over one thousand samples has been frozen over the past four years. DNA was extracted from frozen samples and PCR was used in order to amplify specific polymorphic loci. A hybrid primer technique was employed in order to reduce the cost of labeling the products with fluorescent dyes. The PCR products were checked on agarose gels and analyzed using the CEQ 8000 automated DNA analysis system from Beckman-Coulter. Fragments sizes were determined and genotypes identified using internal reference standards. We are also currently developing an independent data set using RAPD markers that will allow us to confirm and extend our findings. The preliminary results indicate that these techniques will provide a virtually unlimited source of genetic markers for the study of natural populations of zebra mussels in the Great Lakes. Such data will not only be useful in assessing population structure, but should also prove to be invaluable in the evaluation of bioremediation efforts.
[Abstract (DOC)]